Difference between revisions of "Part:BBa K4619003"

Revision as of 16:38, 9 October 2023

TcpP-LBD

This part codes for TcpP ligand binding domain(TcpP-LBD). TcpP-LBD is a single-domain protein that binds the human primary bile acid and could form a dimer when bound. This process is achieved by rewiring TcpP–TcpH from *V. cholerae* to *E.coli* and fusing it with the CadC DNA binding domain to construct a chimeric receptor. When activated, TcpP-CadC forms a dimer and activates the pCadBA promoter, turning on the quorum-sensing cascade. Since TcpP LBD is a single-domain protein, it could be easily swapped into other ligand binding domains like nanobodies, promoting compatibility for the platform.

Sequence and Features