Difference between revisions of "Part:BBa K4619001"

Revision as of 15:17, 9 October 2023

PobR

PobR is a protein that functions as a transcriptional activator. It first attaches to the pobR operator [1]on double-stranded DNA and then interacts with p-hydroxybenzoic acid. This interaction triggers the transcription process from the pobA side of the dual-directional pobA/R promoter [2].One of its benefits is that even small amounts of p-Hydroxybenzoic acid (at the micromolar level) can activate transcription without much leakage.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 462
Illegal AgeI site found at 154
Illegal AgeI site found at 675
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 595

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 <partinfo>BBa_K4619001 short</partinfo>
-PobR is a protein that functions as a transcriptional activator. It first attaches to the pobR operator ([https://parts.igem.org/Part:BBa_K4271007 BBa_K4271007]on double-stranded DNA and then interacts with p-hydroxybenzoic acid. This interaction triggers the transcription process from the pobA side of the dual-directional pobA/R promoter([https://parts.igem.org/Part:BBa_K4271005 BBa_K4271005].One of its benefits is that even small amounts of p-Hydroxybenzoic acid (at the micromolar level) can activate transcription without much leakage.
+PobR is a protein that functions as a transcriptional activator. It first attaches to the pobR operator [https://parts.igem.org/Part:BBa_K4271007BBa_K4271007]on double-stranded DNA and then interacts with p-hydroxybenzoic acid. This interaction triggers the transcription process from the pobA side of the dual-directional pobA/R promoter [https://parts.igem.org/Part:BBa_K4271005BBa_K4271005].One of its benefits is that even small amounts of p-Hydroxybenzoic acid (at the micromolar level) can activate transcription without much leakage.
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