Difference between revisions of "Part:BBa K4724013:Design"
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===Source=== | ===Source=== | ||
| − | It is a mutant of | + | It is a mutant of <i>Is</i>PETase from <i>Ideonella sakaiensis</i>. |
===References=== | ===References=== | ||
Latest revision as of 14:51, 9 October 2023
IsPETaseW159H-6xHis Tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 56
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 56
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 790
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 56
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 56
Illegal AgeI site found at 546 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We needed to purify the IsPETase protein, so we attached the 6xHis tag after IsPETase.
Source
It is a mutant of IsPETase from Ideonella sakaiensis.