Difference between revisions of "Part:BBa K203107:Design"
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===Source=== | ===Source=== | ||
| − | + | Addgene, Cambridge (USA):Plasmid 11979, pJC6-GL3 | |
===References=== | ===References=== | ||
Revision as of 10:43, 19 October 2009
c-Jun promoter (growth factor regulated)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 398
Illegal BamHI site found at 392 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 110
Design Notes
- The plasmid with the promoter inserts was ordered from Addgene, Cambridge (USA):Plasmid 11979, pJC6-GL3
- We used the following primers with BBb_standard for the amplification and isolation of the c-Jun promoter:
- primer forward: 5'-GATCGAATTCGCGGCCGCACTAGTGCATGCCAATTCGAGCTCGG-3'
- primer reverse: 5'-GATCCTGCAGCGGCCGCGCTAGCATCCAGATCTGGATCCAAGCAG-3'
Source
Addgene, Cambridge (USA):Plasmid 11979, pJC6-GL3