Difference between revisions of "Part:BBa K4724006"
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<partinfo>BBa_K4724006 short</partinfo>
 
<partinfo>BBa_K4724006 short</partinfo>
  
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We inserted glutamate E between amino acids 93 and 94 of IsPETase.We chose this site because that the active pocket amino acids are changed, altering the tertiary structure of the protein.
+
We inserted glutamate E between amino acids 93 and 94 of <i>Is</i>PETase.We chose this site because that the active pocket amino acids are changed, altering the tertiary structure of the protein.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:11, 9 October 2023


IsPETaseS93_I94insE-6xHis Tag

We inserted glutamate E between amino acids 93 and 94 of IsPETase.We chose this site because that the active pocket amino acids are changed, altering the tertiary structure of the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 56
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 793
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 56
    Illegal AgeI site found at 549
  • 1000
    COMPATIBLE WITH RFC[1000]