Difference between revisions of "Part:BBa K203106:Design"

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===Design Notes===
 
===Design Notes===
  
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* The plasmid with the promoter inserts was ordered from Addgene, Cambridge (USA): Plasmid 14947: pHMGCS-Luc (aka: pES8)
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* The plasmid with the promoter inserts was ordered from Addgene, Cambridge (USA): Plasmid 14947, pHMGCS-Luc (aka: pES8)
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* We used the following primers with BBb_standard for the amplification and isolation of the LDL receptor promoter:  
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* We used the following primers with BBb_standard for the amplification and isolation of the HMG CoA synthase promoter:  
 
** primer forward: 5'-GATCGAATTCGCGGCCGCACTAGTCGATCTCCTCTCACTGACCTTC-3'  
 
** primer forward: 5'-GATCGAATTCGCGGCCGCACTAGTCGATCTCCTCTCACTGACCTTC-3'  
 
** primer reverse: 5'-GATCCTGCAGGCGGCCGCGCTAGCGCAGCTTTATAGCCACCGACCC-3'
 
** primer reverse: 5'-GATCCTGCAGGCGGCCGCGCTAGCGCAGCTTTATAGCCACCGACCC-3'

Latest revision as of 10:42, 19 October 2009

HMG-CoA synthase promoter (sterol regulated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • The plasmid with the promoter inserts was ordered from Addgene, Cambridge (USA): Plasmid 14947, pHMGCS-Luc (aka: pES8)
  • We used the following primers with BBb_standard for the amplification and isolation of the HMG CoA synthase promoter:
    • primer forward: 5'-GATCGAATTCGCGGCCGCACTAGTCGATCTCCTCTCACTGACCTTC-3'
    • primer reverse: 5'-GATCCTGCAGGCGGCCGCGCTAGCGCAGCTTTATAGCCACCGACCC-3'

Source

Addgene, Cambridge (USA): Plasmid 14947: pHMGCS-Luc (aka: pES8).

References

Castoreno, A. B. et al. Transcriptional regulation of phagocytosis-induced membrane biogenesis by sterol regulatory element binding proteins. Proc Natl Acad Sci U S A 102, 13129–13134 (2005).