Difference between revisions of "Part:BBa K4765129"
Siliang Zhan (Talk | contribs) |
Siliang Zhan (Talk | contribs) |
||
| Line 12: | Line 12: | ||
We use this composite part to test the following stem-loops' ability to prevent mRNA degradation. | We use this composite part to test the following stem-loops' ability to prevent mRNA degradation. | ||
nsl: <pre>5-AAACACCCACCACAAUUUCCACCGUUU UUUGU-3</pre> | nsl: <pre>5-AAACACCCACCACAAUUUCCACCGUUU UUUGU-3</pre> | ||
| − | liu2023 <ref>Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ACS Synthetic Biology, 12(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416</ref>: <pre> 5-AAACACCCACCACAAUUUCCACCGUUU CCCGACGCUUCGGCGUCGGG UUUGU-3</pre> | + | liu2023 <ref>Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ACS Synthetic Biology, 12(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416</ref>: <pre>5-AAACACCCACCACAAUUUCCACCGUUU CCCGACGCUUCGGCGUCGGG UUUGU-3</pre> |
| − | new2: <pre> 5-AAACACCCACCACAAUUUCCACCGUUU CCCCGUCGGCUGCU UUUGU-3</pre> | + | new2: <pre>5-AAACACCCACCACAAUUUCCACCGUUU CCCCGUCGGCUGCU UUUGU-3</pre> |
new6: <pre>5-AAACACCCACCACAAUUUCCACCGUUU AGACGCUCGGCGUCCU UUUGU-3</pre> | new6: <pre>5-AAACACCCACCACAAUUUCCACCGUUU AGACGCUCGGCGUCCU UUUGU-3</pre> | ||
| − | new10: <pre> 5-AAACACCCACCACAAUUUCCACCGUUU ACUGGGGGGAUCGAGGUCUUU UUUGU-3</pre> | + | new10: <pre>5-AAACACCCACCACAAUUUCCACCGUUU ACUGGGGGGAUCGAGGUCUUU UUUGU-3</pre> |
bad2: <pre>5-AAACACCCACCACAAUUUCCACCGUUU GCCGAUCGGGU UUUGU-3</pre> | bad2: <pre>5-AAACACCCACCACAAUUUCCACCGUUU GCCGAUCGGGU UUUGU-3</pre> | ||
| − | bad6: <pre> 5-AAACACCCACCACAAUUUCCACCGUUU AGACGCUCGGCGUCCU UUUGU-3</pre> | + | bad6: <pre>5-AAACACCCACCACAAUUUCCACCGUUU AGACGCUCGGCGUCCU UUUGU-3</pre> |
bad10: <pre>5-AAACACCCACCACAAUUUCCACCGUUU GGCGGCGCUACAGCGUCGU UUUGU-3</pre> | bad10: <pre>5-AAACACCCACCACAAUUUCCACCGUUU GGCGGCGCUACAGCGUCGU UUUGU-3</pre> | ||
Revision as of 12:37, 9 October 2023
stem-loop test
Introduction
This compoiste part is a stem-loop-deleted version of BBa_K4765120, which includes StayGold and mScarlet. Its red-green fluorescence intensity ratio can be compared with BBa_K4765120 to assess the stem-loop's ability to prevent mRNA degradation.
Usage and Biology
We use this composite part to test the following stem-loops' ability to prevent mRNA degradation.
nsl:5-AAACACCCACCACAAUUUCCACCGUUU UUUGU-3liu2023 [1]:
5-AAACACCCACCACAAUUUCCACCGUUU CCCGACGCUUCGGCGUCGGG UUUGU-3new2:
5-AAACACCCACCACAAUUUCCACCGUUU CCCCGUCGGCUGCU UUUGU-3new6:
5-AAACACCCACCACAAUUUCCACCGUUU AGACGCUCGGCGUCCU UUUGU-3new10:
5-AAACACCCACCACAAUUUCCACCGUUU ACUGGGGGGAUCGAGGUCUUU UUUGU-3bad2:
5-AAACACCCACCACAAUUUCCACCGUUU GCCGAUCGGGU UUUGU-3bad6:
5-AAACACCCACCACAAUUUCCACCGUUU AGACGCUCGGCGUCCU UUUGU-3bad10:
5-AAACACCCACCACAAUUUCCACCGUUU GGCGGCGCUACAGCGUCGU UUUGU-3
Characterization
Sequencing map
|
| Figure1 Sequencing map of stem-loop test Sanger sequencing verified we have removed the stem-loop before ribozyme sequence. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1389
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 700
Illegal BsaI.rc site found at 720
Reference
- ↑ Liu, Y., Wu, Z., Wu, D., Gao, N., & Lin, J. (2022). Reconstitution of Multi-Protein Complexes through Ribozyme-Assisted Polycistronic Co-Expression. ACS Synthetic Biology, 12(1), 136–143. https://doi.org/10.1021/acssynbio.2c00416