Difference between revisions of "Part:BBa K4765129"

(→‎Characterization)
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===Introduction===
 
===Introduction===
 
This compoiste part is a stem-loop-deleted version of [https://parts.igem.org/Part:BBa_K4765120 BBa_K4765120], which includes StayGold and mScarlet. Its red-green fluorescence intensity ratio can be compared with [https://parts.igem.org/Part:BBa_K4765120 BBa_K4765120] to assess the stem-loop's ability to prevent mRNA degradation.
 
This compoiste part is a stem-loop-deleted version of [https://parts.igem.org/Part:BBa_K4765120 BBa_K4765120], which includes StayGold and mScarlet. Its red-green fluorescence intensity ratio can be compared with [https://parts.igem.org/Part:BBa_K4765120 BBa_K4765120] to assess the stem-loop's ability to prevent mRNA degradation.
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===Usage and Biology===
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We use this composite part to test the following stem-loops' ability to prevent mRNA degradation.
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nsl:  <pre>5-AAACACCCACCACAAUUUCCACCGUUU UUUGU-3</pre>
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liu2023: <pre> 5-AAACACCCACCACAAUUUCCACCGUUU CCCGACGCUUCGGCGUCGGG UUUGU-3</pre>
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new2:  <pre> 5-AAACACCCACCACAAUUUCCACCGUUU CCCCGUCGGCUGCU UUUGU-3</pre>
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new6:  <pre>5-AAACACCCACCACAAUUUCCACCGUUU AGACGCUCGGCGUCCU UUUGU-3</pre>
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new10:  <pre> 5-AAACACCCACCACAAUUUCCACCGUUU ACUGGGGGGAUCGAGGUCUUU UUUGU-3</pre>
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bad2:  <pre>5-AAACACCCACCACAAUUUCCACCGUUU GCCGAUCGGGU UUUGU-3</pre>
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bad6:  <pre> 5-AAACACCCACCACAAUUUCCACCGUUU AGACGCUCGGCGUCCU UUUGU-3</pre>
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bad10:  <pre>5-AAACACCCACCACAAUUUCCACCGUUU GGCGGCGCUACAGCGUCGU UUUGU-3</pre>
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===Characterization===
 
===Characterization===

Revision as of 12:33, 9 October 2023


stem-loop test contributed by Fudan iGEM 2023


Introduction

This compoiste part is a stem-loop-deleted version of BBa_K4765120, which includes StayGold and mScarlet. Its red-green fluorescence intensity ratio can be compared with BBa_K4765120 to assess the stem-loop's ability to prevent mRNA degradation.

Usage and Biology

We use this composite part to test the following stem-loops' ability to prevent mRNA degradation.

nsl: 
5-AAACACCCACCACAAUUUCCACCGUUU UUUGU-3
liu2023: 
 5-AAACACCCACCACAAUUUCCACCGUUU CCCGACGCUUCGGCGUCGGG UUUGU-3
new2: 
 5-AAACACCCACCACAAUUUCCACCGUUU CCCCGUCGGCUGCU UUUGU-3
new6: 
5-AAACACCCACCACAAUUUCCACCGUUU AGACGCUCGGCGUCCU UUUGU-3
new10: 
 5-AAACACCCACCACAAUUUCCACCGUUU ACUGGGGGGAUCGAGGUCUUU UUUGU-3
bad2: 
5-AAACACCCACCACAAUUUCCACCGUUU GCCGAUCGGGU UUUGU-3
bad6: 
 5-AAACACCCACCACAAUUUCCACCGUUU AGACGCUCGGCGUCCU UUUGU-3
bad10: 
5-AAACACCCACCACAAUUUCCACCGUUU GGCGGCGCUACAGCGUCGU UUUGU-3


Characterization

Sequencing map

contributed by Fudan iGEM 2023
Figure1 Sequencing map of stem-loop test Sanger sequencing verified we have removed the stem-loop before ribozyme sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1389
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 700
    Illegal BsaI.rc site found at 720