Difference between revisions of "Part:BBa K3875019"
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===Usage and Biology=== | ===Usage and Biology=== | ||
It is a bacterial promoter (Registry of Standard Biological Parts BBa_J23119) that modified to end with an SpeI site. J23119 is the "consensus" promoter sequence and the strongest member of the family. The sgRNA will transcribe from the J23119(SpeI) promoter.<br> | It is a bacterial promoter (Registry of Standard Biological Parts BBa_J23119) that modified to end with an SpeI site. J23119 is the "consensus" promoter sequence and the strongest member of the family. The sgRNA will transcribe from the J23119(SpeI) promoter.<br> | ||
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| + | == iGEM23_SCUT-China == | ||
| + | In 2023, SCUT-China successfully applied the CRISPRi system containing this promoter in a novel strain of Streptomyces sp. SCUT-3 and tested effective. Details of the usage can be found in BBa_K4621080. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 12:23, 9 October 2023
Usage and Biology
It is a bacterial promoter (Registry of Standard Biological Parts BBa_J23119) that modified to end with an SpeI site. J23119 is the "consensus" promoter sequence and the strongest member of the family. The sgRNA will transcribe from the J23119(SpeI) promoter.
iGEM23_SCUT-China
In 2023, SCUT-China successfully applied the CRISPRi system containing this promoter in a novel strain of Streptomyces sp. SCUT-3 and tested effective. Details of the usage can be found in BBa_K4621080.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 30
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal SpeI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 30
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 30
- 1000COMPATIBLE WITH RFC[1000]