Difference between revisions of "Part:BBa K203106"

(→‎Usage and Biology)
(→‎Functional Parameters)
Line 16: Line 16:
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K203106 parameters</partinfo>
 
<partinfo>BBa_K203106 parameters</partinfo>
 +
 +
The natural HMG CoA synthase promoter was induced by Lipoprotein Deficient Serum (more details: see Cell Culture Notebook) in HeLa cells. Promoter activtiy was then roughly characterized analogous to REU by TECAN (automated plate fluorescence reader). For more natural promoters look at Part:BBa_K203105 and Part:BBa_K203107.
  
 
{| class="wikitable centered" border="0" rules="rows" style="border-color:white;"
 
{| class="wikitable centered" border="0" rules="rows" style="border-color:white;"
 
|-
 
|-
−
|[[Image:|thumb|none|350px|'''BBa_K203106 is a natural HMG CoA synthase promoter induced approximately by ?% by Lipoprotein Deficient Serum;'''. [http://2009.igem.org/Team:Heidelberg/Measurement REU] was determined by TECAN, 1 experiment à three replicates]]
+
|[[Image:|thumb|none|350px|'''BBa_K203106 is a natural HMG CoA synthase promoter induced approximately 35% by Lipoprotein Deficient Serum;. The GFP flourescence of the HMG CoA synthase promoter was measured by TECAN once (à three replicates). Background fluorescence was substracted, and fluorescence levels are plotted relative to Part:BBa_K203112.]]
 
|}
 
|}
  
  
 
<!-- -->
 
<!-- -->

Revision as of 10:01, 19 October 2009

HMG-CoA synthase promoter (sterol regulated)

This part is a natural promoter responsive to...... and when placed upstream of a gene makes it regulatable by the elements to which this promoter is sensitive.


Usage and Biology

3-hydroxy-3-methylglutaryl-CoA synthase (HMG CoA synthase) is an enzyme catalyzing the condensation of Acetyl-CoA and acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). There are two distinct HMG CoA synthases, the cytosolic and the mitochondrial form, encoded by two different genes. The reaction catalyzed by the cytosolic enzyme is a part of the biosynthesis of cholesterol. In mitochondria the same reaction is responsible for keton body formation. Sterol regulatory elements in the promoter region of the gene are responsible for transcriptional regulation. See reference at [http://2009.igem.org/Team:Heidelberg/Eucaryopedia#Proteins Eucaryopedia].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

n/aHMG-CoA synthase promoter (sterol regulated)

The natural HMG CoA synthase promoter was induced by Lipoprotein Deficient Serum (more details: see Cell Culture Notebook) in HeLa cells. Promoter activtiy was then roughly characterized analogous to REU by TECAN (automated plate fluorescence reader). For more natural promoters look at Part:BBa_K203105 and Part:BBa_K203107.

[[Image:|thumb|none|350px|BBa_K203106 is a natural HMG CoA synthase promoter induced approximately 35% by Lipoprotein Deficient Serum;. The GFP flourescence of the HMG CoA synthase promoter was measured by TECAN once (à three replicates). Background fluorescence was substracted, and fluorescence levels are plotted relative to Part:BBa_K203112.]]