Difference between revisions of "Part:BBa K203105:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | * The plasmid with the promoter inserts was ordered from Addgene, Cambridge (USA): Plasmid 14940, pLDLR-Luc (aka: pES7). | ||
+ | * We used the following primers with BBb_standard for the amplification and isolation of the LDL receptor promoter: | ||
+ | ** primer forward: 5'-GATCGAATTCGCGGCCGCACTAGTAGCCCAATTCGCCCTTGGC-3' | ||
+ | ** primer reverse: 5'-GATCCTGCAGCGGCCGCGCTAGCTTCGCCCTTGGCCCACGTC-3 | ||
===Source=== | ===Source=== |
Revision as of 09:42, 19 October 2009
LDL receptor promoter (sterol regulated)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 139
Illegal SapI site found at 128
Design Notes
- The plasmid with the promoter inserts was ordered from Addgene, Cambridge (USA): Plasmid 14940, pLDLR-Luc (aka: pES7).
- We used the following primers with BBb_standard for the amplification and isolation of the LDL receptor promoter:
- primer forward: 5'-GATCGAATTCGCGGCCGCACTAGTAGCCCAATTCGCCCTTGGC-3'
- primer reverse: 5'-GATCCTGCAGCGGCCGCGCTAGCTTCGCCCTTGGCCCACGTC-3
Source
PCR amplified from Human genomic DNA.