Difference between revisions of "Part:BBa K4765103"

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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html>
 
<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html>
 
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===Introduction===
 
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We introduced a self-assembly synthetic adhesion system by transfecting intimin-Ag2 fusion into ''E. coli''. Intimin-Ag2 fusion is composed of a surface display system(intimin) and the coding sequence of an antigen. The surface display system, which includes a short N-terminal signal peptide to direct its trafficking to the periplasm, a LysM domain for peptidoglycan binding, and a beta-barrel for transmembrane insertion<ref>Piñero-Lambea, C., Bodelón, G., Fernández-Periáñez, R., Cuesta, A. M., Álvarez-Vallina, L., & Fernández, L. Á. (2015). Programming controlled adhesion of ''E. coli'' to target surfaces, cells, and tumors with synthetic adhesins. ''ACS Synthetic Biology, 4''(4), 463–473. https://doi.org/10.1021/sb500252a </ref> , possesses the outer membrane anchoring of the antigen<ref>Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. ''Cell, 174''(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041</ref>.
 
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We’ve constructed this fusion protein into our ribozyme-assisted polycistronic co-expression system：pRAP.
 
  
 
===Usage and Biology===
 
===Usage and Biology===
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The surface-displayed antigen can specifically interact with the nanobody produced by [https://parts.igem.org/Part:BBa_K4765104 BBa_K4765104(ribozyme+strong RBS+intimin-Nb2 fusion+stem-loop)] In our project, we took full advantage of the Ag-Nb interaction to create a biofilm with a programmable physical structure<ref>Kim, H., Skinner, D. J., Glass, D. S., Hamby, A. E., Stuart, B. A. R., Dunkel, J., & Riedel-Kruse, I. H. (2022). 4-bit adhesion logic enables universal multicellular interface patterning. ''Nature, 608''(7922), 324–329. https://doi.org/10.1038/s41586-022-04944-2</ref>..
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Similar to [https://parts.igem.org/Part:BBa_K4765105 BBa_K4765105], we replaced Ag3 with Ag2 in order to interact with [https://parts.igem.org/Part:BBa_K4765102 BBa_K4765104] to create a biofilm.
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===Characterization===
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Revision as of 09:44, 9 October 2023


Twister P1 + T7_RBS + intimin-Ag2 fusion + stem-loop

contributed by Fudan iGEM 2023

Usage and Biology

Similar to BBa_K4765105, we replaced Ag3 with Ag2 in order to interact with BBa_K4765104 to create a biofilm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1305


Reference