Difference between revisions of "Part:BBa K203105"

Revision as of 09:10, 19 October 2009

LDL receptor promoter (sterol regulated)

This part is an natural LDL receptor responsive element which when placed upstream of other genes could induce their transcription in response to lipid addition to the medium.

Usage and Biology

Low-density lipoprotein receptor (LDL receptor) is a cell surface protein which is responsible for the cholesterol supply of the cell. The receptor recognizes the protein B100 which is part of the LDL particles. Binding of the B100 protein to the LDL receptor leads to endocytosis via clathrin coated pits. The vesicle fuses with an endosome, the resulting shift in the pH value leads to the detachment of the LDL. The receptor can be transported back to the plasma membrane (receptor recycling). Through this process the cell takes up the cholesterol which is associated with LDL. LDL accumulation in the blood is responsible for Atherosclerosis and many cardiovascular diseases. The LDL gene is regulated by the intracellular level of cholesterol. A low level of cholesterol leads to the activation whereas a high level results in a decrease of transcription. See reference at [http://2009.igem.org/Team:Heidelberg/Eucaryopedia#Proteins Eucaryopedia].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 139
Illegal SapI site found at 128

Functional Parameters

n/a	LDL receptor promoter (sterol regulated)

[[Image:\|thumb\|none\|350px\|BBa_K203105 is a natural LDL receptor promoter induced approximately by ?% by Lipoprotein Deficient Serum;. [http://2009.igem.org/Team:Heidelberg/Measurement REU] was determined by TECAN, 1 experiment à three replicates]]

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 ===Usage and Biology===
-Low-density lipoprotein receptor (LDL receptor) is a cell surface protein which is responsible for the cholesterol supply of the cell. The receptor recognizes the protein B100 which is part of the LDL particles. Binding of the B100 protein to the LDL receptor leads to endocytosis via clathrin coated pits. The vesicle fuses with an endosome, the resulting shift in the pH value leads to the detachment of the LDL. The receptor can be transported back to the plasma membrane (receptor recycling). Through this process the cell takes up the cholesterol which is associated with LDL. LDL accumulation in the blood is responsible for Atherosclerosis and many cardiovascular diseases. The LDL gene is regulated by the intracellular level of cholesterol. A low level of cholesterol leads to the activation whereas a high level results in a decrease of transcription.
+Low-density lipoprotein receptor (LDL receptor) is a cell surface protein which is responsible for the cholesterol supply of the cell. The receptor recognizes the protein B100 which is part of the LDL particles. Binding of the B100 protein to the LDL receptor leads to endocytosis via clathrin coated pits. The vesicle fuses with an endosome, the resulting shift in the pH value leads to the detachment of the LDL. The receptor can be transported back to the plasma membrane (receptor recycling). Through this process the cell takes up the cholesterol which is associated with LDL. LDL accumulation in the blood is responsible for Atherosclerosis and many cardiovascular diseases. The LDL gene is regulated by the intracellular level of cholesterol. A low level of cholesterol leads to the activation whereas a high level results in a decrease of transcription. See reference at [http://2009.igem.org/Team:Heidelberg/Eucaryopedia#Proteins Eucaryopedia].
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 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K203105 SequenceAndFeatures</partinfo>
 ===Functional Parameters===