Difference between revisions of "Part:BBa K4645001"

Revision as of 06:56, 9 October 2023

NeuA: scFv ( single-chain fragment variable）that inhibits the immune response to the feline

Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies. Among the resulting polyclonal antibodies, a monoclonal antibody named REGN1908 was found to be the most effective in blocking the immune response to the recombinant dimeric Fel D1. Through a literature review, we discovered both light chain and heavy chain genes of REGN1908. By fusing the C-terminal of the light chain with the N-terminal of the heavy chain using a rigid linker (GGGGS)3, an scFv was obtained. An OmpA signal peptide was fused to the N terminal of the scFv to secrete it into the periplasmic space, where it can more effectively neutralize the antigen.

Protein Molecular Structures

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Figure 1. Protein molecular structures of NeuA when it bind to FelD. The blue part is NeuA.

Design

Materials and Method

Result

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 315
Illegal SpeI site found at 75
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 315
Illegal SpeI site found at 75
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 315
Illegal XhoI site found at 693
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 315
Illegal SpeI site found at 75
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 315
Illegal SpeI site found at 75
1000
COMPATIBLE WITH RFC[1000]

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 <center><img src="https://static.igem.wiki/teams/4645/wiki/neua-neub/neua-bind-to-feld.jpg" style="width:40%; "></center>
-<center><b>Figure 1.</b>  Protein molecular structures of NeuA when it bind to FelD.  </center>
+<br>
+<center><b>Figure 1.</b>  Protein molecular structures of NeuA when it bind to FelD. The blue part is NeuA. </center>
 <br>
 </body>