Difference between revisions of "Part:BBa K4645001"
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===Protein Molecular Structures=== | ===Protein Molecular Structures=== | ||
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+ | <center><img src="https://static.igem.wiki/teams/4645/wiki/neua-neub/neua-bind-to-feld.jpg" style="width:40%; "></center> | ||
+ | <center><b>Figure 1.</b> Protein molecular structures of NeuA when it bind to FelD. </center> | ||
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===Design=== | ===Design=== | ||
===Materials and Method=== | ===Materials and Method=== |
Revision as of 06:53, 9 October 2023
NeuA: scFv ( single-chain fragment variable)that inhibits the immune response to the feline
Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies. Among the resulting polyclonal antibodies, a monoclonal antibody named REGN1908 was found to be the most effective in blocking the immune response to the recombinant dimeric Fel D1. Through a literature review, we discovered both light chain and heavy chain genes of REGN1908. By fusing the C-terminal of the light chain with the N-terminal of the heavy chain using a rigid linker (GGGGS)3, an scFv was obtained. An OmpA signal peptide was fused to the N terminal of the scFv to secrete it into the periplasmic space, where it can more effectively neutralize the antigen.
Protein Molecular Structures
Design
Materials and Method
Result
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 315
Illegal SpeI site found at 75 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 315
Illegal SpeI site found at 75 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 315
Illegal XhoI site found at 693 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 315
Illegal SpeI site found at 75 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 315
Illegal SpeI site found at 75 - 1000COMPATIBLE WITH RFC[1000]