Difference between revisions of "Part:BBa K4613016:Design"
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===References=== | ===References=== | ||
+ | Panavas T, Sanders C, Butt T R.SUMO fusion technology for enhanced protein production in prokaryotic and eukaryotic expression systems[J].Methods Mol Biol,2009, 497: 303-17. |
Latest revision as of 06:51, 9 October 2023
SUMO-Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 101
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
By construction of a fusion protein, M-CPA, with SUMO-tag, we increased the solube expression rate of M-CPA in Escherichia coli.
Source
Drosophila melanogaster
References
Panavas T, Sanders C, Butt T R.SUMO fusion technology for enhanced protein production in prokaryotic and eukaryotic expression systems[J].Methods Mol Biol,2009, 497: 303-17.