Difference between revisions of "Part:BBa K4645002"

 
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<partinfo>BBa_K4645002 short</partinfo>
 
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Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies. Among the resulting polyclonal antibodies, a monoclonal antibody named REGN1909 was found to be the most effective in blocking the immune response to the recombinant dimeric Fel D1. Through a literature review, it was discovered that REGN1909 had both light chain and heavy chain genes. By fusing the C terminal of the light chain with the N terminal of the heavy chain using a rigid linker (GGGGS)3, an scFv was obtained. An OmpA signal peptide was fused to the N terminal of the scFv to secrete it into the periplasmic space, where it can more effectively neutralize the antigen.
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Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies. Among the resulting polyclonal antibodies, a monoclonal antibody named REGN1909 was found to be the most effective in blocking the immune response to the recombinant dimeric Fel D1. Through a literature review, we discovered both light chain and heavy chain genes of REGN1909. By fusing the C terminal of the light chain with the N terminal of the heavy chain using a rigid linker (GGGGS)3, an scFv was obtained. An OmpA signal peptide was fused to the N terminal of the scFv to secrete it into the periplasmic space, where it can more effectively neutralize the antigen.
  
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===Usage and Biology===
 
  
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===Protein Molecular Structures===
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===Design===
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===Materials and Method===
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===Result===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
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<partinfo>BBa_K4645002 parameters</partinfo>
 
<partinfo>BBa_K4645002 parameters</partinfo>
 
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Revision as of 04:56, 9 October 2023


NeuB: scFv ( single-chain fragment variable）that inhibits the immune response to the feline

Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies. Among the resulting polyclonal antibodies, a monoclonal antibody named REGN1909 was found to be the most effective in blocking the immune response to the recombinant dimeric Fel D1. Through a literature review, we discovered both light chain and heavy chain genes of REGN1909. By fusing the C terminal of the light chain with the N terminal of the heavy chain using a rigid linker (GGGGS)3, an scFv was obtained. An OmpA signal peptide was fused to the N terminal of the scFv to secrete it into the periplasmic space, where it can more effectively neutralize the antigen.


Protein Molecular Structures

Design

Materials and Method

Result

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 679
    Illegal XhoI site found at 276
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 679
    Illegal SpeI site found at 75
  • 1000
    COMPATIBLE WITH RFC[1000]