Difference between revisions of "Part:BBa K4585004"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 03:24, 9 October 2023

9XUAS

UAS is a piece of small DNA sequence that can be recognized and combined by GAL4. In our design, we have made some alterations. We have repeated the UAS before promoter for 9 times. The purpose of constructing 9×GAL4UAS is to enhance the recognition and combination of GAL4 and to improve the efficiency of downstream target gene activation.

1 Diagrams


Fig 1. The model diagram of 9×UAS

2 Caution

Because we have repeated UAS nine times, we should pay attention to avoiding duplicate fragments when designing primer. If you can't avoid duplicate clips, please note the times that UAS in PCR products has been repeated.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 158
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 158
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 158
    Illegal XhoI site found at 38
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 158
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 158
  • 1000
    COMPATIBLE WITH RFC[1000]