Difference between revisions of "Part:BBa K4613013:Design"

 
 
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===Design Notes===
 
===Design Notes===
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We fused M-CPA into T3 to immobilize the enzyme and increase the stability and sustainable production of M-CPA.
+
We fused M-CPA into T3 to immobilize the enzyme and increase the stability and sustainable production of M-CPA. Codon optimization of M-CPA for enhanced Escherichia coli expression.
  
  

Latest revision as of 02:56, 9 October 2023


T3-M-CPA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 995
    Illegal BamHI site found at 940
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 8


Design Notes

We fused M-CPA into T3 to immobilize the enzyme and increase the stability and sustainable production of M-CPA. Codon optimization of M-CPA for enhanced Escherichia coli expression.


Source

NAU-CHINA 2023

References