Difference between revisions of "Part:BBa K4585003"

 
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<partinfo>BBa_K4585003 short</partinfo>
 
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It belongs to the N-terminal domain of zinc finger protein and binds to some DNA binding domains to inhibit transcription.
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It belongs to the N-terminal domain of zinc finger protein and binds to some DNA binding domains to inhibit transcription. The KRAB domain is a protein-protein interaction domain which can bind to a variety of synergistic transcriptional inhibitors and transcription factors, so that KRAB zinc finger proteins as transcriptional factors or transcriptional regulators plays a transcriptional inhibitory function dependently on DNA binding.
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                <!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title-->
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                1.Diagrams
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                    <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/krab.png"></p>
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1  The model diagram of KRAB</p>
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                    <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/krabdian.png"></p>
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Agarose gel electrophoresis map of KRAB fragment PCR product</p>
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                2.Caution
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                <p>Different KRAB domains have different silencing effects on target genes, regardless of cell types. The KRAB protein domain must be linked to the DNA binding domains to play an inhibitory role.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:12, 9 October 2023

KRAB

It belongs to the N-terminal domain of zinc finger protein and binds to some DNA binding domains to inhibit transcription. The KRAB domain is a protein-protein interaction domain which can bind to a variety of synergistic transcriptional inhibitors and transcription factors, so that KRAB zinc finger proteins as transcriptional factors or transcriptional regulators plays a transcriptional inhibitory function dependently on DNA binding.

1.Diagrams

Fig.1 The model diagram of KRAB

Fig.2 Agarose gel electrophoresis map of KRAB fragment PCR product

2.Caution

Different KRAB domains have different silencing effects on target genes, regardless of cell types. The KRAB protein domain must be linked to the DNA binding domains to play an inhibitory role.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 178