Difference between revisions of "Part:BBa K4585001"
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<p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Agarose gel electrophoresis map of GAL4 fragment PCR product</p> | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Agarose gel electrophoresis map of GAL4 fragment PCR product</p> |
Latest revision as of 01:59, 9 October 2023
GAL4
GAL4 is a kind of protein that can find and combined with UAS DNA structure domain.It can recognize and combine with UAS. Because GAL4 is originated from yeast cells, the activation domain can not work in mammalian cells. Therefore, we only used its combining domain and use it together with VP64 .
1.Diagrams
Fig.1 The model diagram of GAL4
Fig.2 Agarose gel electrophoresis map of GAL4 fragment PCR product
2.Caution
GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 215
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 134