Difference between revisions of "Part:BBa K4585001"

 
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GAL4 is a kind of protein that can find and combined with UAS DNA structure domain.
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GAL4 is a kind of protein that can find and combined with UAS DNA structure domain.It can recognize and combine with UAS. Because GAL4 is originated from yeast cells, the activation domain can not work in mammalian cells. Therefore, we only used its combining domain and use it together with VP64 .
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                <!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title-->
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                1.Diagrams
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                    <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/gal4.png"></p>
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1  The model diagram of GAL4</p>
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                    <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/gal4.png"></p>
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Agarose gel electrophoresis map of GAL4 fragment PCR product</p>
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                2.Caution
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                <p>GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:58, 9 October 2023


GAL4

GAL4 is a kind of protein that can find and combined with UAS DNA structure domain.It can recognize and combine with UAS. Because GAL4 is originated from yeast cells, the activation domain can not work in mammalian cells. Therefore, we only used its combining domain and use it together with VP64 .

1.Diagrams

Fig.1 The model diagram of GAL4

Fig.2 Agarose gel electrophoresis map of GAL4 fragment PCR product

2.Caution

GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 134