Difference between revisions of "Part:BBa K4593020:Design"
Jianfei Song (Talk | contribs) (→Design Notes) |
Jianfei Song (Talk | contribs) (→Source) |
||
| Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
| + | LysDZ25 is from bacteriophage DZ25[1], LysGH15 is from Bacteriophage GH15[2], and ClyC is a hybrid endolysin with CBD of LysPALS1 and EAD of LysSA12[3]; Spn1s_LysRZ is from bacteriophage SPN1S[4]. | ||
| − | + | The QS system is from the S. aureus genome, contributed by iGEM07_Cambridge. | |
===References=== | ===References=== | ||
Revision as of 23:43, 8 October 2023
S. aureus in vivo elimination apparatus for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 3468
Illegal NheI site found at 3491 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 418
Illegal BglII site found at 6040
Illegal BamHI site found at 1302
Illegal XhoI site found at 2591 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 5684
Illegal NgoMIV site found at 6524
Illegal NgoMIV site found at 7198
Illegal AgeI site found at 3090
Illegal AgeI site found at 3228
Illegal AgeI site found at 5553
Illegal AgeI site found at 6668
Illegal AgeI site found at 6896
Illegal AgeI site found at 7067
Illegal AgeI site found at 7180 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The promoter, RBS, and codon frequency of the circuit are optimized for protein expression in E. coli. However, the P2 promoter shows a high background expression in E. coli, making the device unable to conduct its designated function in E. coli. For a modified version for the expression in B. subtilis, see BBa_K4593021
Source
LysDZ25 is from bacteriophage DZ25[1], LysGH15 is from Bacteriophage GH15[2], and ClyC is a hybrid endolysin with CBD of LysPALS1 and EAD of LysSA12[3]; Spn1s_LysRZ is from bacteriophage SPN1S[4].
The QS system is from the S. aureus genome, contributed by iGEM07_Cambridge.