Difference between revisions of "Part:BBa K5023014"

 
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__NOTOC__
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<partinfo>BBa_K5023012 short</partinfo>
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With the PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the PHL7 enzyme and the terminator. Designed to express and secrete PHL7 in Chlamydomonas reinhardtii, utilizing pJP32 backbone. This circuit is originally designed by our advisor João Molino.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5023014 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K5023014 parameters</partinfo>
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Revision as of 21:26, 8 October 2023


PAR_Ble_SP7_FAST-PETase_Linker_MHETase_SP20_Ter

With the PAR promoter, the bleomycin/zeocin resistance sequence, signal peptide, the PHL7 enzyme and the terminator. Designed to express and secrete PHL7 in Chlamydomonas reinhardtii, utilizing pJP32 backbone. This circuit is originally designed by our advisor João Molino.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 698
    Illegal PstI site found at 1572
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal NheI site found at 1211
    Illegal PstI site found at 698
    Illegal PstI site found at 1572
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2043
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 698
    Illegal PstI site found at 1572
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 698
    Illegal PstI site found at 1572
    Illegal NgoMIV site found at 1079
    Illegal NgoMIV site found at 1140
    Illegal NgoMIV site found at 1357
    Illegal NgoMIV site found at 1458
    Illegal NgoMIV site found at 2370
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1808