Difference between revisions of "Part:BBa K215107"

Revision as of 01:41, 19 October 2009

Extracellular Secretion System

This part is made of 3 genes: prtD, prtE, and prtF, that constitute a type I Erwinia chrysanthemi secretion system. The operon is expressed from a strong constitutive promoter, BBa_J23100, and has the translational terminator BBa_B0014. In PSB3T5.

Used to secrete proteins containing prtB C-terminal tag. The prtB C-terminal tag is built into the protein generator [BBa_K215002]. Any protein of interest can be inserted into the protein generator and then secreted when used in conjunction with this secretion system.

Usage and Biology

To test this secretion system's functionality, it was used to secrete [BBa_K215010] (GFP fusion protein containing prtB at C-terminus).

[Image:Secretion_data_plot2.png]

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  TargetGFP in Media jjjjjjjs.

Sequence and Features