Difference between revisions of "Part:BBa K215010"
 
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<partinfo>BBa_K215010 short</partinfo>
 
<partinfo>BBa_K215010 short</partinfo>
  
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This part is GFP with an N-terminal tag that binds to streptavidin and a C-terminal tag that signals for secretion to the extracellular space (when combined with proper secretion system BioBrick BBa_K215107). Its expression is IPTG inducible.
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This part is GFP (E0040) amplified out and inserted into K215002 at the NheI cut site then put into the expression vector K215000.  For information about this parts functionality when expressed please see [https://parts.igem.org/wiki/index.php?title=Part:BBa_K215002 BBa_K215002] page or the [http://2009.igem.org/Team:Washington University of Washington 2009 project Wiki].
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:36, 19 October 2009

pLac+RBS+GFP with Secretion Signal and Streptavidin Binding Tags on GFP

This part is GFP (E0040) amplified out and inserted into K215002 at the NheI cut site then put into the expression vector K215000. For information about this parts functionality when expressed please see BBa_K215002 page or the [http://2009.igem.org/Team:Washington University of Washington 2009 project Wiki].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 842