Difference between revisions of "Part:BBa K4880006:Design"

 
(Design Notes)
 
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===Design Notes===
 
===Design Notes===
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+
In our design, the expression of bisabolene synthase is controlled by IPTG and theophylline. Transcription is regulated by IPTG and translation initiates only when there is theophylline present.
 
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+
  
 
===Source===
 
===Source===

Latest revision as of 17:29, 8 October 2023


Ptrc-theo-AgBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1842
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1842
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1437
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1842
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1842
    Illegal AgeI site found at 55
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 225
    Illegal BsaI.rc site found at 666
    Illegal BsaI.rc site found at 1449


Design Notes

In our design, the expression of bisabolene synthase is controlled by IPTG and theophylline. Transcription is regulated by IPTG and translation initiates only when there is theophylline present.

Source

This composite part combines BBa_K3930029 and BBa_K4880002.

References