Difference between revisions of "Part:BBa K4613209"

Revision as of 17:03, 8 October 2023

Molecular Beacon

The locked hairpin is composed of the sequence of Ochratoxin A (OTA) aptamer, the sequence of RCA primer and the locked sequence. In the absence of OTA, the locked hairpin should be stable as "close status", eliminating nonspecific recognition. In the presence of OTA, the hairpin structure would transform into “open” conformation due to the recognition event between the aptamer and OTA.

Fig.1 The principle of locked hairpin structure-switching.

Reference

Zhang J, Lu Y, Gao W, et al. Structure-switching locked hairpin triggered rolling circle amplification for ochratoxin A (OTA) detection by ICP-MS[J]. Microchemical Journal, 2023, 186: 108365.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 16: / Line 16: @@
 ==== Reference ====
-#Xu G, Zhao J, Yu H, et al. Structural insights into the mechanism of high-affinity binding of ochratoxin A by a DNA aptamer[J]. Journal of the American Chemical Society, 2022, 144(17): 7731-7740.
 #Zhang J, Lu Y, Gao W, et al. Structure-switching locked hairpin triggered rolling circle amplification for ochratoxin A (OTA) detection by ICP-MS[J]. Microchemical Journal, 2023, 186: 108365.