Difference between revisions of "Part:BBa K4585006"

 
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We used homologous recombination method to construct plasmids, pcDNA3.1(+)-3×HA-GAL4-VP64-NLS linearized vector can undergo homologous recombination with GAL4 fragment (BBa_K4585001) and VP64 fragment to obtain pcDNA3.1(+)-3×HA-GAL4-VP64-NLS plasmid (BBa_K4585012).
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                Diagrams
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                    <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/fig-1-the-agarose-gel-electrophoresis-of-pcdna3-1-3-ha-gal4-vp64-nls-linearized-vector.png"></p>
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig 1. The agarose gel electrophoresis of pcDNA3.1(+)-3×HA-GAL4-VP64-NLS linearized vector</p>
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Revision as of 15:47, 8 October 2023

pcDNA3.1(+)-3XHA-GAL4-VP64-NLS linearized vector

We used homologous recombination method to construct plasmids, pcDNA3.1(+)-3×HA-GAL4-VP64-NLS linearized vector can undergo homologous recombination with GAL4 fragment (BBa_K4585001) and VP64 fragment to obtain pcDNA3.1(+)-3×HA-GAL4-VP64-NLS plasmid (BBa_K4585012).

Diagrams


Fig 1. The agarose gel electrophoresis of pcDNA3.1(+)-3×HA-GAL4-VP64-NLS linearized vector