Difference between revisions of "Part:BBa K4806215:Design"
| Line 26: | Line 26: | ||
<li>3. Connector B5-B6 (<a href=" https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>)<sup>*</sup></li> | <li>3. Connector B5-B6 (<a href=" https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>)<sup>*</sup></li> | ||
<li>4. Connector F1-F2 (<a href=" https://parts.igem.org/Part:BBa_K4806018">BBa_K4806018</a>)</li> | <li>4. Connector F1-F2 (<a href=" https://parts.igem.org/Part:BBa_K4806018">BBa_K4806018</a>)</li> | ||
| − | <li> | + | <li>5. Connector F2-F3 (<a href=" https://parts.igem.org/Part:BBa_K4806019">BBa_K4806019</a>)</li> |
| − | <li> | + | <li>6. Connector F3-F4 (<a href=" https://parts.igem.org/Part:BBa_K4806020">BBa_K4806020</a>)</li> |
| − | <li> | + | <li>7. Connector F4-F5 (<a href=" https://parts.igem.org/Part:BBa_K4806021">BBa_K4806021</a>)</li> |
| − | <li> | + | <li>8. Connector F5-F6 (<a href=" https://parts.igem.org/Part:BBa_K4806021">BBa_K4806021</a>)</li> |
| − | <li> | + | <li>9. Dummy for F3 (<a href=" https://parts.igem.org/Part:BBa_K4806017">BBa_K4806017</a>)</li> |
| − | <li> | + | <li>10. Dummy for F5 (<a href=" https://parts.igem.org/Part:BBa_K4806022">BBa_K4806022</a>)</li> |
| − | <li> | + | <li>11. Endlinker (<a href=" https://parts.igem.org/Part:BBa_K4806016">BBa_K4806016</a>)</li> |
</ul> | </ul> | ||
</p> | </p> | ||
Latest revision as of 14:58, 8 October 2023
POR/CYP2D6 tandem with HA-tag for Chlamydomonas reinhardtii (Phytobrick)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3722
Illegal PstI site found at 4982
Illegal PstI site found at 5042
Illegal PstI site found at 5701
Illegal PstI site found at 8141
Illegal PstI site found at 9497
Illegal PstI site found at 9930 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2618
Illegal NheI site found at 4907
Illegal NheI site found at 7292
Illegal PstI site found at 3722
Illegal PstI site found at 4982
Illegal PstI site found at 5042
Illegal PstI site found at 5701
Illegal PstI site found at 8141
Illegal PstI site found at 9497
Illegal PstI site found at 9930
Illegal NotI site found at 5347
Illegal NotI site found at 8647 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2899
Illegal XhoI site found at 7573 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3722
Illegal PstI site found at 4982
Illegal PstI site found at 5042
Illegal PstI site found at 5701
Illegal PstI site found at 8141
Illegal PstI site found at 9497
Illegal PstI site found at 9930 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3722
Illegal PstI site found at 4982
Illegal PstI site found at 5042
Illegal PstI site found at 5701
Illegal PstI site found at 8141
Illegal PstI site found at 9497
Illegal PstI site found at 9930
Illegal NgoMIV site found at 1270
Illegal NgoMIV site found at 1452
Illegal NgoMIV site found at 6630
Illegal NgoMIV site found at 8784
Illegal NgoMIV site found at 9133
Illegal NgoMIV site found at 10474
Illegal AgeI site found at 2637
Illegal AgeI site found at 7311 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.
Source
This composite part is a combination of the following parts:
- 1. AβSAP(i)-promotor (A1-B2) (BBa_K4806013)
- 2.1 CYP3A4 gene (B3-B4) (BBa_K4806000)
- 2.2 POR gene (B3-B4) (BBa_K4806003)
- 3. HA-tag (B5) (BBa_K3002017)*
- 4. tRPL23-terminator (B6-C1) (BBa_K3002006)*
- 1. Connector B2-B3 (BBa_K3002303)*
- 2. Connector B4-B5 (BBa_K3002304)*
- 3. Connector B5-B6 (BBa_K3002305)*
- 4. Connector F1-F2 (BBa_K4806018)
- 5. Connector F2-F3 (BBa_K4806019)
- 6. Connector F3-F4 (BBa_K4806020)
- 7. Connector F4-F5 (BBa_K4806021)
- 8. Connector F5-F6 (BBa_K4806021)
- 9. Dummy for F3 (BBa_K4806017)
- 10. Dummy for F5 (BBa_K4806022)
- 11. Endlinker (BBa_K4806016)
The * marked parts were not created by us. Our results can be found on the experience page of each part.