Difference between revisions of "Part:BBa K4806215:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
a
+
This part has been codon optimized for <i>Chlamydomonas reinhardtii</i> and it is compatible with Phytobricks/MoClo parts.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
−
 
+
<html>
−
a
+
<p>
 +
This composite part is a combination of the following parts:
 +
<ul>
 +
<li>1. AβSAP(i)-promotor (A1-B2) (<a href=" https://parts.igem.org/Part:BBa_K4806013">BBa_K4806013</a>)</li>
 +
<li>2.1 CYP3A4 gene (B3-B4) (<a href=" https://parts.igem.org/Part:BBa_K4806000">BBa_K4806000</a>)</li>
 +
<li>2.2 POR gene (B3-B4) (<a href=" https://parts.igem.org/Part:BBa_K4806003">BBa_K4806003</a>)</li>
 +
<li>3. HA-tag (B5)  (<a href=" https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>)<sup>*</sup></li>
 +
<li>4. tRPL23-terminator (B6-C1) (<a href=" https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>)<sup>*</sup></li>
 +
</ul>
 +
Additionally, connectors were used to create the MoClo overhangs:
 +
<ul>
 +
<li>1. Connector B2-B3 (<a href=" https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>)<sup>*</sup></li>
 +
<li>2. Connector B4-B5 (<a href=" https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>)<sup>*</sup></li>
 +
<li>3. Connector B5-B6 (<a href=" https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>)<sup>*</sup></li>
 +
<li>4. Connector F1-F2 (<a href=" https://parts.igem.org/Part:BBa_K4806018">BBa_K4806018</a>)</li>
 +
<li>3. Connector F2-F3 (<a href=" https://parts.igem.org/Part:BBa_K4806019">BBa_K4806019</a>)</li>
 +
<li>3. Connector F3-F4 (<a href=" https://parts.igem.org/Part:BBa_K4806020">BBa_K4806020</a>)</li>
 +
<li>3. Connector F4-F5 (<a href=" https://parts.igem.org/Part:BBa_K4806021">BBa_K4806021</a>)</li>
 +
<li>3. Connector F5-F6 (<a href=" https://parts.igem.org/Part:BBa_K4806021">BBa_K4806021</a>)</li>
 +
<li>3. Dummy for F3 (<a href=" https://parts.igem.org/Part:BBa_K4806017">BBa_K4806017</a>)</li>
 +
<li>3. Dummy for F5 (<a href=" https://parts.igem.org/Part:BBa_K4806022">BBa_K4806022</a>)</li>
 +
<li>3. Endlinker (<a href=" https://parts.igem.org/Part:BBa_K4806016">BBa_K4806016</a>)</li>
 +
</ul>
 +
</p>
 +
<p>The <sup>*</sup> marked parts were not created by us. Our results can be found on the experience page of each part.</p>
 +
</html>
  
 
===References===
 
===References===

Revision as of 14:57, 8 October 2023


POR/CYP2D6 tandem with HA-tag for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3722
    Illegal PstI site found at 4982
    Illegal PstI site found at 5042
    Illegal PstI site found at 5701
    Illegal PstI site found at 8141
    Illegal PstI site found at 9497
    Illegal PstI site found at 9930
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2618
    Illegal NheI site found at 4907
    Illegal NheI site found at 7292
    Illegal PstI site found at 3722
    Illegal PstI site found at 4982
    Illegal PstI site found at 5042
    Illegal PstI site found at 5701
    Illegal PstI site found at 8141
    Illegal PstI site found at 9497
    Illegal PstI site found at 9930
    Illegal NotI site found at 5347
    Illegal NotI site found at 8647
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2899
    Illegal XhoI site found at 7573
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3722
    Illegal PstI site found at 4982
    Illegal PstI site found at 5042
    Illegal PstI site found at 5701
    Illegal PstI site found at 8141
    Illegal PstI site found at 9497
    Illegal PstI site found at 9930
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3722
    Illegal PstI site found at 4982
    Illegal PstI site found at 5042
    Illegal PstI site found at 5701
    Illegal PstI site found at 8141
    Illegal PstI site found at 9497
    Illegal PstI site found at 9930
    Illegal NgoMIV site found at 1270
    Illegal NgoMIV site found at 1452
    Illegal NgoMIV site found at 6630
    Illegal NgoMIV site found at 8784
    Illegal NgoMIV site found at 9133
    Illegal NgoMIV site found at 10474
    Illegal AgeI site found at 2637
    Illegal AgeI site found at 7311
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

Additionally, connectors were used to create the MoClo overhangs:

The * marked parts were not created by us. Our results can be found on the experience page of each part.

References