Difference between revisions of "Part:BBa K4806214:Design"
| Line 14: | Line 14: | ||
This composite part is a combination of the following parts: | This composite part is a combination of the following parts: | ||
<ul> | <ul> | ||
| − | <li>1. | + | <li>1. AβSAP(i)-promotor (A1-B2) (<a href=" https://parts.igem.org/Part:BBa_K4806013">BBa_K4806013</a>)</li> |
| − | <li> | + | <li>2.1 CYP3A4 gene (B3-B4) (<a href=" https://parts.igem.org/Part:BBa_K4806000">BBa_K4806000</a>)</li> |
| − | <li>2. POR gene (B3-B4) (<a href=" https://parts.igem.org/Part:BBa_K4806003">BBa_K4806003</a>)</li> | + | <li>2.2 POR gene (B3-B4) (<a href=" https://parts.igem.org/Part:BBa_K4806003">BBa_K4806003</a>)</li> |
<li>3. HA-tag (B5) (<a href=" https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>)<sup>*</sup></li> | <li>3. HA-tag (B5) (<a href=" https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>)<sup>*</sup></li> | ||
<li>4. tRPL23-terminator (B6-C1) (<a href=" https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>)<sup>*</sup></li> | <li>4. tRPL23-terminator (B6-C1) (<a href=" https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>)<sup>*</sup></li> | ||
Revision as of 14:10, 8 October 2023
POR/CYP3A4 tandem with HA-tag for Chlamydomonas reinhardtii (Phytobrick)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3722
Illegal PstI site found at 4982
Illegal PstI site found at 5042
Illegal PstI site found at 5701
Illegal PstI site found at 8870
Illegal PstI site found at 9192
Illegal PstI site found at 9252 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2618
Illegal NheI site found at 4907
Illegal NheI site found at 7273
Illegal PstI site found at 3722
Illegal PstI site found at 4982
Illegal PstI site found at 5042
Illegal PstI site found at 5701
Illegal PstI site found at 8870
Illegal PstI site found at 9192
Illegal PstI site found at 9252
Illegal NotI site found at 5347 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2899
Illegal XhoI site found at 7554 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3722
Illegal PstI site found at 4982
Illegal PstI site found at 5042
Illegal PstI site found at 5701
Illegal PstI site found at 8870
Illegal PstI site found at 9192
Illegal PstI site found at 9252 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3722
Illegal PstI site found at 4982
Illegal PstI site found at 5042
Illegal PstI site found at 5701
Illegal PstI site found at 8870
Illegal PstI site found at 9192
Illegal PstI site found at 9252
Illegal NgoMIV site found at 1270
Illegal NgoMIV site found at 1452
Illegal NgoMIV site found at 6630
Illegal NgoMIV site found at 9114
Illegal NgoMIV site found at 10618
Illegal AgeI site found at 2637
Illegal AgeI site found at 7292 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.
Source
This composite part is a combination of the following parts:
- 1. AβSAP(i)-promotor (A1-B2) (BBa_K4806013)
- 2.1 CYP3A4 gene (B3-B4) (BBa_K4806000)
- 2.2 POR gene (B3-B4) (BBa_K4806003)
- 3. HA-tag (B5) (BBa_K3002017)*
- 4. tRPL23-terminator (B6-C1) (BBa_K3002006)*
- 1. Connector B1-B2 (BBa_K3002302)*
- 2. Connector B2-B3 (BBa_K3002303)*
- 3. Connector B4-B5 (BBa_K3002304)*
- 4. Connector B5-B6 (BBa_K3002305)*
The * marked parts were not created by us. Our results can be found on the experience page of each part.