Difference between revisions of "Part:BBa K4665171:Design"

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===Source===
 
===Source===
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The helper plasmid was constructed by Praetorius et al., (2017) by cloning the coding parts of helper phage M13KO7 into a pSC101 plasmid backbone carrying a kanamycin resistance gene.
  
 
===References===
 
===References===
  
 
Praetorius, F., Kick, B., Behler, K. et al. (2017). Biotechnological mass production of DNA origami.Nature 552, 84–87. https://doi.org/10.1038/nature24650
 
Praetorius, F., Kick, B., Behler, K. et al. (2017). Biotechnological mass production of DNA origami.Nature 552, 84–87. https://doi.org/10.1038/nature24650

Revision as of 12:12, 8 October 2023


HP17_KO7


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5958
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

add later


Source

The helper plasmid was constructed by Praetorius et al., (2017) by cloning the coding parts of helper phage M13KO7 into a pSC101 plasmid backbone carrying a kanamycin resistance gene.

References

Praetorius, F., Kick, B., Behler, K. et al. (2017). Biotechnological mass production of DNA origami.Nature 552, 84–87. https://doi.org/10.1038/nature24650