Difference between revisions of "Part:BBa K4665171"
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When using the helper-plasmid approach, the pScaf containing the DNA-origami sequence (<b>BBa_K4665170</b>) and the helper plasmid are both introduced into the bacteria by simultaneous transformation. The helper plasmid contains the origin of replication to be able to produce the ssDNA sequence needed for DNA origami. | When using the helper-plasmid approach, the pScaf containing the DNA-origami sequence (<b>BBa_K4665170</b>) and the helper plasmid are both introduced into the bacteria by simultaneous transformation. The helper plasmid contains the origin of replication to be able to produce the ssDNA sequence needed for DNA origami. | ||
| − | The helper plasmid was constructed by Praetorius et al.,(2017) by cloning the coding parts of helper phage M13KO7 into a pSC101 plasmid backbone carrying a kanamycin resistance gene. | + | The helper plasmid was constructed by Praetorius et al., (2017) by cloning the coding parts of helper phage M13KO7 into a pSC101 plasmid backbone carrying a kanamycin resistance gene. |
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Latest revision as of 12:09, 8 October 2023
HP17_KO7
Biology and Usage
The helper plasmid is part of a system to create ssDNA using bacteria. This can be used for DNA origami.
When using the helper-plasmid approach, the pScaf containing the DNA-origami sequence (BBa_K4665170) and the helper plasmid are both introduced into the bacteria by simultaneous transformation. The helper plasmid contains the origin of replication to be able to produce the ssDNA sequence needed for DNA origami.
The helper plasmid was constructed by Praetorius et al., (2017) by cloning the coding parts of helper phage M13KO7 into a pSC101 plasmid backbone carrying a kanamycin resistance gene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 5958
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Praetorius, F., Kick, B., Behler, K. et al. (2017). Biotechnological mass production of DNA origami.Nature 552, 84–87. https://doi.org/10.1038/nature24650