Difference between revisions of "Part:BBa K200022"

 
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<partinfo>BBa_K200022 short</partinfo>
 
<partinfo>BBa_K200022 short</partinfo>
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Ligation of heat repressible regulation system to GFP to assay its activity.
 
Ligation of heat repressible regulation system to GFP to assay its activity.
  
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This part comprises of the heat sensitive Lamda cI Repressor ([[Part:BBa_K200011 |BBa_K200011]]) with a standard GFP reporter output ([[Part:BBa_I13504 |BBa_I13504]]). This part was used as a test construct, to check the functionality of the part. By measuring GFP flourescence at 28 degrees, 42 degrees and with a temperature shift from 28 to 42 degrees, the part was shown to function.
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===Usage and Biology===
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===Usage and Biology===
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The Imperial iGEM 2009 Team successfully used this construct to show the functionality of this composite part. By measuring the increase in GFP flourescence over a temperature shift, the part was shown to be working successfully.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 22:09, 18 October 2009

Heat-inducible system with GFP reporter

Ligation of heat repressible regulation system to GFP to assay its activity.

This part comprises of the heat sensitive Lamda cI Repressor (BBa_K200011) with a standard GFP reporter output (BBa_I13504). This part was used as a test construct, to check the functionality of the part. By measuring GFP flourescence at 28 degrees, 42 degrees and with a temperature shift from 28 to 42 degrees, the part was shown to function.


Usage and Biology

The Imperial iGEM 2009 Team successfully used this construct to show the functionality of this composite part. By measuring the increase in GFP flourescence over a temperature shift, the part was shown to be working successfully. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1599