Difference between revisions of "Part:BBa K4665170"

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<span class='h3bb'>===Sequence and Features===</span>
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<span class='h3bb'><b>Sequence and Features</b></span>
 
<partinfo>BBa_K4665170 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4665170 SequenceAndFeatures</partinfo>
  

Revision as of 09:11, 8 October 2023


Octahedron DNA-origami (3024bp long; 42bp edges)

Biology and Usage

DNA origami is a technique that allows for the fabrication of complex nanostructures through guided DNA folding (Majikes & Liddle, 2021). The process involves designing a long ssDNA molecule, referred to as a scaffold, which acts as a backbone for the assembly. The long strand of ssDNA will then be folded into the wanted format using short ssDNA fragments called staples. Almost any form can be achieved by altering the staples. To get the ssDNA sequence a pScaf phagemid with insert for generating DNA origami was used.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 919
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 919
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 738
    Illegal BglII site found at 1310
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 919
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 919
    Illegal NgoMIV site found at 132
  • 1000
    COMPATIBLE WITH RFC[1000]


References