Difference between revisions of "Part:BBa K4623001:Design"

Revision as of 09:04, 8 October 2023

Monomeric streptavidin(mSA)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 82
Illegal AgeI site found at 142
1000
COMPATIBLE WITH RFC[1000]

Design Notes

We have performed codon optimization on the obtained amino acid sequence.

Source

We got the sequence from https://pubmed.ncbi.nlm.nih.gov/22806584/

References

Lim KH, Huang H, Pralle A, Park S. Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection. Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. Epub 2012 Aug 8. PMID: 22806584.

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	===References===		===References===
		+	Lim KH, Huang H, Pralle A, Park S. Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection. Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. Epub 2012 Aug 8. PMID: 22806584.