Difference between revisions of "Part:BBa K200018"
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This part contains a GFP (green fluorescent protein) reporter in the functional transcriptional unit. As such, activity of the pCstA promoter can be assessed by the expression of GFP.
 
This part contains a GFP (green fluorescent protein) reporter in the functional transcriptional unit. As such, activity of the pCstA promoter can be assessed by the expression of GFP.
  
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===Usage and Biology===
 
===Usage and Biology===
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This construct was used by Imperial College's 2009 iGEM team [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project. It was ligated to [https://parts.igem.org/Part:BBa_J5526 BBa_J5526] to produce the final construct [https://parts.igem.org/wiki/index.php/Part:BBa_K200019 BBa_K200019].
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K200018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K200018 SequenceAndFeatures</partinfo>

Revision as of 20:42, 18 October 2009

pCstA+RBS+GFP+TT

This part contains a GFP (green fluorescent protein) reporter in the functional transcriptional unit. As such, activity of the pCstA promoter can be assessed by the expression of GFP.


Usage and Biology

This construct was used by Imperial College's 2009 iGEM team [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project. It was ligated to BBa_J5526 to produce the final construct BBa_K200019.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 801