Difference between revisions of "Part:BBa K4891005"

Revision as of 07:28, 8 October 2023

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It is the key gene element for constructing shikimate biosynthetic pathway. Using this recombinant plasmid, shikimate could be generated from phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) by multi-enzyme cascade reaction.

Usage and Biology

Characterization

2023 Hangzhou-SDG Team characterized this part as a suicide switch We tested the suicide switches based on the amounts of spore formation and the spore germination rates. Engineered strains were inoculated to the larvae of the greater wax moth (Galleria mellonella). The Petri dishes containing the pest corpses were placed under sunshine for days until spores formed outside the insect body. Spores were collected by vertexing and the concentrations of spore suspensions were checked under a microscope.

< img src = "https://static.igem.wiki/teams/4891/wiki/parts/1.png" style = "width:500px">

Figure 1. A. The amount of spore formation by each strain on G. mellonella corpses; B. The spore germination rate of each strain in liquid PDB media after 10h. *: p < 0.01; **: p < 0.001.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2175
Illegal XhoI site found at 934
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1965
Illegal AgeI site found at 2509
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2362
Illegal SapI.rc site found at 241

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 It is the key gene element for constructing shikimate biosynthetic pathway. Using this recombinant plasmid, shikimate could be generated from phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) by multi-enzyme cascade reaction.
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
 ===Characterization===