Difference between revisions of "Part:BBa K190035:Design"

(→‎Source)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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The Melbourne 2007 <partinfo>BBa_I750016</partinfo> GVP biobrick was cloned behind a copper inducible promoter.
+
Melbourne GVP cluster was used containing the 400bp repeat in gvpL.
  
 
===Source===
 
===Source===

Latest revision as of 18:57, 18 October 2009

GVP with CueO regulated promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 385
    Illegal BamHI site found at 4190
    Illegal XhoI site found at 3878
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 1238
    Illegal AgeI site found at 4157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2648
    Illegal SapI.rc site found at 3804


Design Notes

Melbourne GVP cluster was used containing the 400bp repeat in gvpL.

Source

E.coli and Bacillus megaterium

References