Difference between revisions of "Part:BBa K4591002"
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===ZJUT-China 2023's Contribution===
 
===ZJUT-China 2023's Contribution===
 
===Usage and Biology===
 
===Usage and Biology===
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<p><i>XylS</i> is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding and protein dimerization. XylS detect benzoate and its derivatives, mainly methyl and chlorine substitution at 2-, 3- carbon<sup>[1]</sup>.</p>
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<p><i>XylS</i> is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding an`d protein dimerization. [1]</p>
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<p>In this way, the Xyl could active the <i>Pm</i> promotor by recognizes two 15-bp repeats (TGCA-N6-GGNTA) in <i>Pm</i> promoter when the benzoate and its derivatives was detected. </p>
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<p>XylS can bind benzoic acid and various derivatives,
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but it cannot recognize PA and TPA.[2-5] So the Jiawei Li and Mario Roque Huanca Nina successfully make the directed evolution of XylS to generate new TFs that are capable of binding and responding to TPA and PA . Such XylS mutants could be used to construct whole-cell biosensors for fluorimetric detection of PA and TPA. </p>
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<p>The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua.</p>
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===photo1 from ShiMing===
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<p>BBa_K3202001 When the TPA was detected by the Xylsmut, the P<sub>m</sub> promotor would be actived and transcript the downstream fragments.[6]</p>
 +
<p>In this year, we have designed a system of PET detection, attachment and degradation.In order to detect the 
 +
<p>The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua.It was</p>
  
  

Revision as of 05:42, 8 October 2023


XylSmut

ZJUT-China 2023's Contribution

Usage and Biology

XylS is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding an`d protein dimerization. [1]

XylS can bind benzoic acid and various derivatives, but it cannot recognize PA and TPA.[2-5] So the Jiawei Li and Mario Roque Huanca Nina successfully make the directed evolution of XylS to generate new TFs that are capable of binding and responding to TPA and PA . Such XylS mutants could be used to construct whole-cell biosensors for fluorimetric detection of PA and TPA.

photo1 from ShiMing

BBa_K3202001 When the TPA was detected by the Xylsmut, the Pm promotor would be actived and transcript the downstream fragments.[6]

In this year, we have designed a system of PET detection, attachment and degradation.In order to detect the <p>The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua.It was


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]