Difference between revisions of "Part:BBa K4591002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
<p><i>XylS</i> is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding and protein dimerization. XylS detect benzoate and its derivatives, mainly methyl and chlorine substitution at 2-, 3- carbon<sup>[1]</sup>.</p> | <p><i>XylS</i> is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding and protein dimerization. XylS detect benzoate and its derivatives, mainly methyl and chlorine substitution at 2-, 3- carbon<sup>[1]</sup>.</p> | ||
| − | <p>In this way, the Xyl could active the <i>Pm</i> promotor by recognizes two 15-bp repeats (TGCA-N6-GGNTA) in <i>Pm</i> promoter. </p> | + | <p>In this way, the Xyl could active the <i>Pm</i> promotor by recognizes two 15-bp repeats (TGCA-N6-GGNTA) in <i>Pm</i> promoter when the benzoate and its derivatives was detected. </p> |
<p>The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua.</p> | <p>The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua.</p> | ||
Revision as of 05:11, 8 October 2023
XylSmut
ZJUT-China 2023's Contribution
Usage and Biology
XylS is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding and protein dimerization. XylS detect benzoate and its derivatives, mainly methyl and chlorine substitution at 2-, 3- carbon[1].
In this way, the Xyl could active the Pm promotor by recognizes two 15-bp repeats (TGCA-N6-GGNTA) in Pm promoter when the benzoate and its derivatives was detected.
The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]