Difference between revisions of "Part:BBa K4765129"
Siliang Zhan (Talk | contribs) (→Introduction) |
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===Characterization=== | ===Characterization=== | ||
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| + | | <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/stem-loop-test-sequence.png" alt="contributed by Fudan iGEM 2023"></html> | ||
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| + | | '''Figure1 Sequencing map of stem-loop test''' Sanger sequencing verified we have removed the stem-loop before ribozyme sequence. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 03:25, 8 October 2023
stem-loop test
Introduction
This compoiste part is a stem-loop-deleted version of BBa_K4765120, which includes StayGold and mScarlet. Its red-green fluorescence intensity ratio can be compared with BBa_K4765120 to assess the stem-loop's ability to prevent mRNA degradation.
Characterization
Sequencing map
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| Figure1 Sequencing map of stem-loop test Sanger sequencing verified we have removed the stem-loop before ribozyme sequence. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1389
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 700
Illegal BsaI.rc site found at 720