Difference between revisions of "Part:BBa K4765025"

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| <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/sequencing-map-of-rv-dsup.jpg" alt="contributed by Fudan iGEM 2023"></html>
 
| <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/sequencing-map-of-rv-dsup.jpg" alt="contributed by Fudan iGEM 2023"></html>
 
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| '''Figure1 sequencing map of Rv Dsup''' Sequencing starts from the T7 terminator, with the primer 5-GCTAGTTATTGCTCAGCGG-3.
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| '''Figure1 Sequencing map of Rv Dsup''' Sequencing starts from the T7 terminator, with the primer 5-GCTAGTTATTGCTCAGCGG-3.
 
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Revision as of 03:01, 8 October 2023


Rv Dsup codon optimized

contributed by Fudan iGEM 2023

Introduction

Rv Dsup is a damage suppressor protein from the Tardigrade Species Ramazzottius Varieornatus [1] . This is a nucleosome-binding protein that protects chromatin from hydroxyl radicals[2] , thereby inhibiting DNA molecule breakage. This protein can reduce X-ray-induced damage in human cells by 40%.

Usage and Biology

We optimized the codons of wildtype Rv Dsup for E. coli K12 and heterologously expressed it in E. coli to enhance its survival rate under UV radiation.

Characterization

Sequencing map

contributed by Fudan iGEM 2023
Figure1 Sequencing map of Rv Dsup Sequencing starts from the T7 terminator, with the primer 5-GCTAGTTATTGCTCAGCGG-3.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 169
    Illegal AgeI site found at 240
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 166
    Illegal SapI.rc site found at 895


Reference

  1. ↑ Hashimoto, T., Horikawa, D. D., Saito, Y., Kuwahara, H., Kozuka-Hata, H., Shin-I, T., Minakuchi, Y., Ohishi, K., Motoyama, A., Aizu, T., Enomoto, A., Kondo, K., Tanaka, S., Hara, Y., Koshikawa, S., Sagara, H., Miura, T., Yokobori, S. I., Miyagawa, K., Suzuki, Y., … Kunieda, T. (2016). Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nature communications, 7, 12808. https://doi.org/10.1038/ncomms12808
  2. ↑ Chavez, C., Cruz-Becerra, G., Fei, J., Kassavetis, G. A., & Kadonaga, J. T. (2019). The tardigrade damage suppressor protein binds to nucleosomes and protects DNA from hydroxyl radicals. eLife, 8, e47682. https://doi.org/10.7554/eLife.47682