Difference between revisions of "Part:BBa K4591002"
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===ZJUT-China 2023's Contribution===
 
===ZJUT-China 2023's Contribution===
 
===Usage and Biology===
 
===Usage and Biology===
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<p>XylS is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding and protein dimerization<sup>[1]</sup>. XylS detect benzoate and its derivatives, mainly methyl and chlorine substitution at 2-, 3- carbon<sup>[2]</sup>.</p>
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<p><i>XylS</i> is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding and protein dimerization. XylS detect benzoate and its derivatives, mainly methyl and chlorine substitution at 2-, 3- carbon<sup>[1]</sup>.</p>
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<p>The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua,and the Team Peking iGEM2013 has charcterized the part and some of its mutations.The result shows that </p>
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<p>In this way, the Xyl could active the <i>Pm</i> promotor by recognizes two 15-bp repeats (TGCA-N6-GGNTA) in <i>Pm</i> promoter. </p>
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<p>The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua.</p>
  
  

Revision as of 02:54, 8 October 2023


XylSmut

ZJUT-China 2023's Contribution

Usage and Biology

XylS is an archetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-terminal domain (CTD) involved in DNA binding, and an N-terminal domain required for effector binding and protein dimerization. XylS detect benzoate and its derivatives, mainly methyl and chlorine substitution at 2-, 3- carbon[1].

In this way, the Xyl could active the Pm promotor by recognizes two 15-bp repeats (TGCA-N6-GGNTA) in Pm promoter.

The xylsmut is upgraded form an existing part Xyls(BBa_K108029)from the Team iGEM_Tsinghua.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]