Difference between revisions of "Part:BBa K220000:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K220000 short</partinfo>
 
<partinfo>BBa_K220000 short</partinfo>
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'''Fwd:''' 5'- AAC GAA TTC TAC TCT AGA AGG AGG ATA ATT TAA TGA GAG TGG AAA ATA GCA ACG GA-3'
 
'''Fwd:''' 5'- AAC GAA TTC TAC TCT AGA AGG AGG ATA ATT TAA TGA GAG TGG AAA ATA GCA ACG GA-3'
  
−
'''Length:'' 33   
+
'''Length:''' 33   
 
'''Rev:''' 5'- ACC ACT AGT CTA CTA TAT TTT GTC GGA CTT GCG-3'
 
'''Rev:''' 5'- ACC ACT AGT CTA CTA TAT TTT GTC GGA CTT GCG-3'
  

Revision as of 18:11, 18 October 2009

Sarcoisine Dimethyl Glycine Methyl Transferase (SDMT)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 777
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 777
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 71
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 777
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 777
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 836


Design Notes

Length: 56 Fwd: 5'- AAC GAA TTC TAC TCT AGA AGG AGG ATA ATT TAA TGA GAG TGG AAA ATA GCA ACG GA-3'

Length: 33 Rev: 5'- ACC ACT AGT CTA CTA TAT TTT GTC GGA CTT GCG-3'



Source

Gene isolation from Galdieria sulphuraria

References