Difference between revisions of "Part:BBa K185033"

 
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<partinfo>BBa_K185033 short</partinfo>
 
<partinfo>BBa_K185033 short</partinfo>
  
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It is an inverter which its downsteam gene will always be repressed by lacI protein unless IPTG is added.
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And the synthetic rate of the downsteam gene is low because the low binding efficiency of B0031.
  
 
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Revision as of 17:42, 18 October 2009

An inverter of a special lactose operon system based on J23110-rbs34-lacI-dter-plac-rbs31

It is an inverter which its downsteam gene will always be repressed by lacI protein unless IPTG is added. And the synthetic rate of the downsteam gene is low because the low binding efficiency of B0031.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]