Difference between revisions of "Part:BBa K4757999"

 
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Expression cassette consinsting of XylS-K38R-L224Q/Pm promoter, activated by terepthalic acid, and AlkS-V760E/pAlkB activated by n-dodecane.
 
Expression cassette consinsting of XylS-K38R-L224Q/Pm promoter, activated by terepthalic acid, and AlkS-V760E/pAlkB activated by n-dodecane.
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Gene expression controlled by the pAlkB promoter can be degraded with sRNA interference, expressed with Pm promoter
+
Gene expression controlled by the pAlkB promoter can be degraded with sRNA interference, expressed with Pm promoter. test 123
  
 
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Revision as of 19:39, 7 October 2023


Synthetic expression cassette regulated by terepthalic acid and alkanes for PET and PE sensing

Expression cassette consinsting of XylS-K38R-L224Q/Pm promoter, activated by terepthalic acid, and AlkS-V760E/pAlkB activated by n-dodecane. Gene expression controlled by the pAlkB promoter can be degraded with sRNA interference, expressed with Pm promoter. test 123

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4202
    Illegal EcoRI site found at 4298
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4202
    Illegal EcoRI site found at 4298
    Illegal NotI site found at 1625
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4202
    Illegal EcoRI site found at 4298
    Illegal BglII site found at 212
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4202
    Illegal EcoRI site found at 4298
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4202
    Illegal EcoRI site found at 4298
    Illegal NgoMIV site found at 934
    Illegal NgoMIV site found at 2716
    Illegal NgoMIV site found at 3187
  • 1000
    COMPATIBLE WITH RFC[1000]