Difference between revisions of "Part:BBa K4613202"

 
 
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<partinfo>BBa_K4613003 short</partinfo>
  
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The locked hairpin is composed of the sequence of Ochratoxin A(OTA) aptamer, the sequence of RCA primer and the locked sequence. In the absence of OTA, the locked hairpin should be stable as "close status", eliminating nonspecific recognition. In the presence of OTA, the hairpin structure would transform into “open” conformation due to the recognition event between the aptamer and OTA.
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<center><img src="https://static.igem.wiki/teams/4613/wiki/parts/locked-hairpin.png"with="1000" height="" width="500" height=""/></center>
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<p style="text-align: center!important;"><b>Fig.1 The principle of locked hairpin structure-switching.</b></p>
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==== Reference ====
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#Zhang J, Lu Y, Gao W, et al. Structure-switching locked hairpin triggered rolling circle amplification for ochratoxin A (OTA) detection by ICP-MS[J]. Microchemical Journal, 2023, 186: 108365.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4613003 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4613003 parameters</partinfo>
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Latest revision as of 17:42, 7 October 2023

Molecular Beacon

The locked hairpin is composed of the sequence of Ochratoxin A(OTA) aptamer, the sequence of RCA primer and the locked sequence. In the absence of OTA, the locked hairpin should be stable as "close status", eliminating nonspecific recognition. In the presence of OTA, the hairpin structure would transform into “open” conformation due to the recognition event between the aptamer and OTA.


Fig.1 The principle of locked hairpin structure-switching.


Reference

  1. Zhang J, Lu Y, Gao W, et al. Structure-switching locked hairpin triggered rolling circle amplification for ochratoxin A (OTA) detection by ICP-MS[J]. Microchemical Journal, 2023, 186: 108365.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]