Difference between revisions of "Part:BBa K200024"
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<partinfo>BBa_K200024 short</partinfo> | <partinfo>BBa_K200024 short</partinfo> | ||
| − | Colanic acid producing gene with double terminator | + | Colanic acid producing gene with double terminator. |
RcsB ([https://parts.igem.org/Part:BBa_K200000:Design BBa_K200000]) coupled to the double terminator ([https://parts.igem.org/Part:BBa_B0015:Design BBa_B0015]). | RcsB ([https://parts.igem.org/Part:BBa_K200000:Design BBa_K200000]) coupled to the double terminator ([https://parts.igem.org/Part:BBa_B0015:Design BBa_B0015]). | ||
Latest revision as of 16:08, 18 October 2009
RcsB+TT
Colanic acid producing gene with double terminator.
RcsB (BBa_K200000) coupled to the double terminator (BBa_B0015).
RcsB overexpression turn on the RCS phosphorelay system. This results in the modulation of ~5% of the E.coli genome. One of the most notable effects of this is the production of the exopolysaccharide colanic acid.
The double terminator is the most commonly used terminator and is a combination of parts (BBa_B0010) and (BBa_B0012).
The double terminator allows the RcsB gene to be incorporated into a closed transcriptional unit.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]